Part:BBa_K2202001
ssDNA for producing in-vivo tetrahedral structure strand2
This part is an improvement to the BBa_K2054002 part. This part works together with four other parts ( BBa_K2202000, BBa_K2202002, BBa_K2202003 and BBa_K2202004) and requires the chassis to contain HIV reverse transcriptase and Murine leukemia reverse transcriptase. The Part essentially produces a ncRNA which is later reverse transcribed to a single-stranded DNA that binds together with four other ssDNA produced to make a 2-dimensional structure. This 2D structure, upon detecting a Huntington's Disease biomarker (hsa-miR-34b-3p), folds into a tetrahedron with a G-quadruplex. Colorimetric or fluorescent reagents such as ABTS and Thioflavin T can be used to detect this G-quadruplex. Hence our part functions as a diagnostic device for nucleic acid based biomarkers.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 146
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 101
Illegal SapI site found at 272
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